Inhibition of microbial lipases by fatty acids.

Production, purification, characterization, and applications of lipases.

Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in nature, but only microbial lipases are commercially significant. The many applications of lipases include speciality organic syntheses, hydrolysis of fats and oils, modification of fats, flavor enhancement in food proce...

Activation and inhibition of Candida rugosa and Bacillus-related lipases by saturated fatty acids, evaluated by a new colorimetric microassay.

Research on lipase inhibitors could help in the therapy of diseases caused by lipase-producing microorganisms and in the design of novel lipase substrate specificities for biotechnology. Here we report a fast and sensitive colorimetric microassay that is low-cost and suitable for high-throughput experiments for the evaluation of lipase activity and inhibition. Comparison of Candida rugosa activ...

Inhibition of Thermophilic Anaerobic Digestion of Waste Food by Long Chain Fatty Acids and Propionate

Activity of microbial lipases on natural fats and synthetic triglycerides.

The positional and fatty acid specificities of lipases from several different microorganisms have been examined by gas-liquid chromatographic analysis of the free fatty acids following action on mixed triglycerides of known composition. Three types of lipases were found. Lipases of the first type, obtained from several microorganisms, attacked primarily the 1-position of the triglyceride in a m...

Characterization of Lipase from Wild (LPF-5) and Mutant (HN1) Strain of Aspergillus niger

Lipases (triacyl glycerol acylhydrolases, EC 3.1.1.3) belonging to the class Hydrolases catalyze hydrolysis of insoluble triacylglycerols (TAGs) to generate free fatty acids, mono and diacylglycerols and glycerol (Das et al., 2016). Lipases are found in both prokaryotes (bacteria and archaea) as well as in eukaryotes (animals, plants and fungi) (Cai-hong et al., 2008). The most broadly applied ...

Inhibition of fatty acids profile changes of Cobia (Rachycentron canadum) fillets during frozen storage by packaging under vacuum system

This study was aimed to investigate the effect of vacuum packing (VP) on the fatty acids profiles in cobia (Rachycentron canadum) fillets during an extended frozen storage period. Cobia fillets were treated under vacuum system then stored at -18°C for up to 6 months and compared to control conditions. As a result of a frozen storage period of 6 months, a marked content decrease was found in the...